Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis).
Identifieur interne : 000277 ( Main/Exploration ); précédent : 000276; suivant : 000278Comparative transcriptome analysis reveals a global insight into molecular processes regulating citrate accumulation in sweet orange (Citrus sinensis).
Auteurs : Xiaopeng Lu [République populaire de Chine] ; Xiongjun Cao [République populaire de Chine] ; Feifei Li [République populaire de Chine] ; Jing Li [République populaire de Chine] ; Jiang Xiong [République populaire de Chine] ; Guiyou Long [République populaire de Chine] ; Shangyin Cao [République populaire de Chine] ; Shenxi Xie [République populaire de Chine]Source :
- Physiologia plantarum [ 1399-3054 ] ; 2016.
Abstract
Citrate, the predominant organic acid in citrus, determines the taste of these fruits. However, little is known about the synergic molecular processes regulating citrate accumulation. Using 'Dahongtiancheng' (Citrus sinensis) and 'Bingtangcheng' (C. sinensis) with significant difference in citrate, the objectives of this study were to understand the global mechanisms of high-citrate accumulation in sweet orange. 'Dahongtiancheng' and 'Bingtangcheng' exhibit significantly different patterns in citrate accumulation throughout fruit development, with the largest differences observed at 50-70 days after full bloom (DAFB). Comparative transcriptome profiling was performed for the endocarps of both cultivars at 50 and 70 DAFB. Over 34.5 million clean reads per library were successfully mapped to the reference database and 670-2630 differentially expressed genes (DEGs) were found in four libraries. Among the genes, five transcription factors were ascertained to be the candidates regulating citrate accumulation. Functional assignments of the DEGs indicated that photosynthesis, the citrate cycle and amino acid metabolism were significantly altered in 'Dahongtiancheng'. Physiological and molecular analyses suggested that high photosynthetic efficiency and partial impairment of citrate catabolism were crucial for the high-citrate trait, and amino acid biosynthesis was one of the important directions for citrate flux. The results reveal a global insight into the gene expression changes in a high-citrate compared with a low-citrate sweet orange. High accumulating efficiency and impaired degradation of citrate may be associated with the high-citrate trait of 'Dahongtiancheng'. Findings in this study increase understanding of the molecular processes regulating citrate accumulation in sweet orange.
DOI: 10.1111/ppl.12484
PubMed: 27507765
Affiliations:
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Changsha</wicri:regionArea><wicri:noRegion>Changsha</wicri:noRegion></affiliation></author><author><name sortKey="Cao, Shangyin" sort="Cao, Shangyin" uniqKey="Cao S" first="Shangyin" last="Cao">Shangyin Cao</name><affiliation wicri:level="1"><nlm:affiliation>Zhengzhou Fruit Research Institute, Chinese academy of Agricultural Sciences, Zhengzhou, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Zhengzhou Fruit Research Institute, Chinese academy of Agricultural Sciences, Zhengzhou</wicri:regionArea><wicri:noRegion>Zhengzhou</wicri:noRegion></affiliation></author><author><name sortKey="Xie, Shenxi" sort="Xie, Shenxi" uniqKey="Xie S" first="Shenxi" last="Xie">Shenxi Xie</name><affiliation wicri:level="1"><nlm:affiliation>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha, China. shenxixie@163.com.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Horticulture, College of Horticulture and Landscape, Hunan Agricultural University, Changsha</wicri:regionArea><wicri:noRegion>Changsha</wicri:noRegion></affiliation></author></analytic><series><title level="j">Physiologia plantarum</title><idno type="eISSN">1399-3054</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Citrate, the predominant organic acid in citrus, determines the taste of these fruits. However, little is known about the synergic molecular processes regulating citrate accumulation. Using 'Dahongtiancheng' (Citrus sinensis) and 'Bingtangcheng' (C. sinensis) with significant difference in citrate, the objectives of this study were to understand the global mechanisms of high-citrate accumulation in sweet orange. 'Dahongtiancheng' and 'Bingtangcheng' exhibit significantly different patterns in citrate accumulation throughout fruit development, with the largest differences observed at 50-70 days after full bloom (DAFB). Comparative transcriptome profiling was performed for the endocarps of both cultivars at 50 and 70 DAFB. Over 34.5 million clean reads per library were successfully mapped to the reference database and 670-2630 differentially expressed genes (DEGs) were found in four libraries. Among the genes, five transcription factors were ascertained to be the candidates regulating citrate accumulation. Functional assignments of the DEGs indicated that photosynthesis, the citrate cycle and amino acid metabolism were significantly altered in 'Dahongtiancheng'. Physiological and molecular analyses suggested that high photosynthetic efficiency and partial impairment of citrate catabolism were crucial for the high-citrate trait, and amino acid biosynthesis was one of the important directions for citrate flux. The results reveal a global insight into the gene expression changes in a high-citrate compared with a low-citrate sweet orange. High accumulating efficiency and impaired degradation of citrate may be associated with the high-citrate trait of 'Dahongtiancheng'. Findings in this study increase understanding of the molecular processes regulating citrate accumulation in sweet orange.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Lu, Xiaopeng" sort="Lu, Xiaopeng" uniqKey="Lu X" first="Xiaopeng" last="Lu">Xiaopeng Lu</name></noRegion><name sortKey="Cao, Shangyin" sort="Cao, Shangyin" uniqKey="Cao S" first="Shangyin" last="Cao">Shangyin Cao</name><name sortKey="Cao, Xiongjun" sort="Cao, Xiongjun" uniqKey="Cao X" first="Xiongjun" last="Cao">Xiongjun Cao</name><name sortKey="Li, Feifei" sort="Li, Feifei" uniqKey="Li F" first="Feifei" last="Li">Feifei Li</name><name sortKey="Li, Jing" sort="Li, Jing" uniqKey="Li J" first="Jing" last="Li">Jing Li</name><name sortKey="Long, Guiyou" sort="Long, Guiyou" uniqKey="Long G" first="Guiyou" last="Long">Guiyou Long</name><name sortKey="Xie, Shenxi" sort="Xie, Shenxi" uniqKey="Xie S" first="Shenxi" last="Xie">Shenxi Xie</name><name sortKey="Xiong, Jiang" sort="Xiong, Jiang" uniqKey="Xiong J" first="Jiang" last="Xiong">Jiang Xiong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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